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ミドリシャミセンガイ Lingula unguis (触手動物,腕足綱)のスフィンゴリン脂質はセラミドホスホエタノールアミンである
http://hdl.handle.net/10441/2455
http://hdl.handle.net/10441/245565307c3a-42d8-4849-b5c1-95f050d68ca8
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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| 公開日 | 2009-04-29 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | ミドリシャミセンガイ Lingula unguis (触手動物,腕足綱)のスフィンゴリン脂質はセラミドホスホエタノールアミンである | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | jpn | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| アクセス権 | ||||||||||||||||||
| アクセス権 | metadata only access | |||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||||||||
| 著者 |
堀, 太郎
× 堀, 太郎
× 坂田, 綾子
× 青木, 一弘
× 林, 陽
× Dulaney, John T.
× 杉田, 陸海
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| 著者(ヨミ) | ||||||||||||||||||
| 姓名 | ホリ, タロウ | |||||||||||||||||
| 著者(ヨミ) | ||||||||||||||||||
| 姓名 | サカタ, アヤコ | |||||||||||||||||
| 著者(ヨミ) | ||||||||||||||||||
| 姓名 | アオキ, カズヒロ | |||||||||||||||||
| 著者(ヨミ) | ||||||||||||||||||
| 姓名 | ハヤシ, アキラ | |||||||||||||||||
| 著者(ヨミ) | ||||||||||||||||||
| 姓名 | スギタ, ムツミ | |||||||||||||||||
| 著者別名 | ||||||||||||||||||
| 姓名 | Hori, Taro | |||||||||||||||||
| 著者別名 | ||||||||||||||||||
| 姓名 | Sakata, Ayako | |||||||||||||||||
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| 姓名 | Aoki, Kazuhiro | |||||||||||||||||
| 著者別名 | ||||||||||||||||||
| 姓名 | Hayashi, Akira | |||||||||||||||||
| 著者別名 | ||||||||||||||||||
| 姓名 | Sugita, Mutsumi | |||||||||||||||||
| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Ceramide phosphoethanolamine has been found to be the principal phosphosphingolipid in the mesogastropodean snails, the dipterous insects and the periodical millipede. The presence of ceramide phosphoethanolamine as a major phospholipid constituent in these organisms is unique, since it has not been found, or is present only in trace amounts in other animals investigated so far. In this paper we describe the isolation and the structural characterization of ceramide phosphoethanolamine from Lingula unguis(phylum Tentaculata, class Brachiopoda), one of the oldest "living fossils". Alkali-stable lipids(2.7g) prepared from the chloroform-methanol extracts of L. unguis were fractionated by two ion-exchange(QAE-and DEAE-Sephadex A-25) column chromatographic media into three types of fractions, namely; Type I : choline-containing phospholipid fraction(1.16g); Type II: ethanolamine-containing phospholipid fraction(780mg); Type III:serine and inositol-containing phospholipid fraction(270mg). The thin layer chromatographic pattern of the Type II fraction revealed the presence of three cornponents(PL-1, PL-2 and PL-3)with positive reactions toward molybdate (phosphate group) and ninhydrin(amino group)spray reagents. These components were separated and purified by silicic acid (Iatrobeads) column chromatography and their homogeneities were determined on thin layer plates developed with chloroformmethanol-water (65:25:4, v/v) and chloroform-methanol-acetic acid-water(100;20:12:5, v/v). The yields were PL-1,92.1mg; PL-2, 5.9mg; and PL-3, 15.1mg, respectively. The infrared spectra of the fast-moving compounds, PL-1 and PL-2 were essentially identical to the published spectra of ceramide phosphoethanolamine, except for a stronger absorption around 3300 cm-1 in PL-2 due to a free hydroxy group. PL-3, with a lower mobility on the thin layer chromatogram, was distinguishable from the others on the basis of its infrared characteristics. These characteristics suggested that PL-3 might be a 1-alkylglycerophospholipid, which may have been formed by deacylation of 1-alkyl-2-acylglycerophospholipid. Acid hydrolysis of PL-1 and/or PL-2 with 1 M HCl at 100℃ for 2 hr yielded both ethanolamine and phosphoethanolamine. The latter was converted to a trimethylsilyl derivative with bis(trimethylsilyl) acetamide, and analyzed by gas chromatography-mass spectrometry. Phosphoethano lamine was identified by reference to the mass spectrum of the authentic compound. Dephosphorylation of three phospholipids with HF yielded ceramides from both PL-1 and PL-2, and 1-alkylglycerol from PL-3. The ceramide moieties were mainly composed of C16:0, C17:0 and C18:0 fatty acids and 4△-C18:1 sphingoid for PL-1, and 2-hydroxy-C16:0, -C17:0 and -C18:0 acids and 4,8,10△-C18:3 sphingoid for PL-2. |
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| 引用 | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | 滋賀大学教育学部紀要, 自然科学・教育科学, 第45号, pp.31-42 | |||||||||||||||||
| 書誌情報 |
滋賀大学教育学部紀要, 自然科学・教育科学 号 45, p. 31-42, 発行日 1996-03-15 |
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| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 1340-7953 | |||||||||||||||||
| 書誌レコードID | ||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AN10064721 | |||||||||||||||||
| タイトル（ヨミ） | ||||||||||||||||||
| その他のタイトル | ミドリシャミセンガイ LINGULA UNGUIS ショクシュ ドウブツ ワンソク コウ ノ スフィンゴリン シシツ ワ セラミド ホスホエタノールアミン デアル | |||||||||||||||||
| その他の言語のタイトル | ||||||||||||||||||
| その他のタイトル | A Main Phosphosphingolipid In Lingula unguis (Phylum Tentaculata, Class Branchiopoda) Is Ceramide Phosphoethanolamine | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | 滋賀大学教育学部 | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | Departmental Bulletin Paper | |||||||||||||||||
