@article{oai:shiga-u.repo.nii.ac.jp:00001768, author = {糸乗, 前 and 北村, 朋典 and 田中, 理恵子 and 宮垣, 紀子 and 齋藤, 洋昭 and 杉田, 陸海}, issue = {56}, journal = {滋賀大学教育学部紀要, 3, 自然科学}, month = {}, note = {Departmental Bulletin Paper, Two phosphonosphingolipids,ceramide aminoethylphosphonates named CAEPn-1 and CAEPn-2 were purified from the pearl oyster,Pinctada martensii by successive column chromatography on ion exchange Sephadex (QAE-and DEAE-Sephadex) and silicic acid (Iatrobeads) and their aliphatic components were determined by converting to various derivatives.The numbers of carbons and hydroxylated positions of the fatty acids were confirmed by gas chromatograph-mass spectrometry as their methyl ester derivatives.On the other hand, the numbers of carbons and double bonds of the sphingoids were confirmed by gas chromatograph-mass spectrometry as their trimethylsilyl ether derivatives.Double bond positions in the aliphatic chains were also confirmed by gas chromatographic and gas chromatograph-mass spectrometric identifications of the fatty acid products after periodate-permanganate oxidation of the ceramides derived from the CAEPn by hydrogen fluoride degradation.The major fatty acids of CAEPn-1 were hexadecanoic (82.0%)and heptadecanoic acid (13.9%), and those of CAEPn-2,2-hydroxyhexadecanoic (84.1%) and 2-hydroxy heptadecanoic acid (15.9%).The major sphingoids of CAEPn-1 were octadeca sphinga-4-enine (24.7%),4,8-dienine(40.1%) and 4,8,10-trienine (24.6%),and those of CAEPn-2,octadeca sphinga-4,8,10-trienine (29.2%)and its 9-methyl homologue (58.6%)., 滋賀大学教育学部紀要, 3, 自然科学, 第56号, pp.51-62}, pages = {51--62}, title = {アコヤガイ, Pinctada martensii のホスホノ脂質(Ceramide 2-Aminoethylphosphonate)のセラミド組成}, year = {2006} }