{"created":"2023-05-15T15:24:06.335898+00:00","id":1740,"links":{},"metadata":{"_buckets":{"deposit":"3ea30882-9b05-47b5-9539-929ccad065e0"},"_deposit":{"created_by":1,"id":"1740","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1740"},"status":"published"},"_oai":{"id":"oai:shiga-u.repo.nii.ac.jp:00001740","sets":["21:22:163:169"]},"author_link":["8200","8204","8206","8207","8202","8203","8201","8208","8205"],"item_2_alternative_title_20":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"アミノトウ ガンユウ スフィンゴ トウシシツ ノ ヒドラジン ブンカイ ブンキ トウサ ノ ケツゴウ イチ ケッテイ エノ オウヨウ"}]},"item_2_alternative_title_22":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Hydrazinolysis of N-Acetylhexosamines-Containing Glycosphingolipids : Application of hydrazinolysis to branching sugar structure analysis"}]},"item_2_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"51","bibliographicPageEnd":"42","bibliographicPageStart":"35","bibliographic_titles":[{"bibliographic_title":"滋賀大学教育学部紀要, 3, 自然科学"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Departmental Bulletin Paper","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In  protostomia,　most  complex  glycosphingolipids  have  N-acetylhexosamine  as  a  sugercomponent　and　there　are　branching　sugars　on　the　core　sequence　in　the　molecules.\n　In　this　branching  structure,　it  is　important　but　difficult　to　determine　which　sugar　attaches　to　which　position  on　the　reducing　end　sugar. 　Based　on　the　knowledge　that　hexsosaminyl　linkage　is　much　more\nresistant　to　acid　cleavage　than　neutral　sugar　linkages (include  N-acetylhaxsosaminyl),　this　linkage  therefore　could　be　distinguished　from　other　glycosyl　bonds.　Hydrazinolysis　is　used　to　selectively  cleave  amide  bonds  in  protein　and　has  been　applied　to  the　synthesis  of  radioactive  derivative　by\nre-acetylation　of  de-N-acetyl  glycosphingolipids.\nIn　this　experiment,　we　examined　whether　controlled　hydrazinolysis　could　be　used　for　de-N-acetylation　of　complex　glycosphingolipids.　Ceramide　tri-,　tetra,　and　pentasaccharides　are  readily　converted　to　their　de-N-acetyl　glycolipid　deriavatives　as　shown　by　TLC　and　MALDI-TOFMS　analysis.　And　we　confirmed　by　GC　analysis　that　hexosaminyl　linkage　are　resistant　to　acid  cleavage.\nFinally,  we  have  applied  this  technique　to  an  example  of  complex  glycosphingolipid,\nnamely　ceramide　nonasaccharide,　and　have　detected　its　complete　sugar　linkager　which　agree　with  its　previously　determined　branching　structure.　This　controlled　hydrazinolysis　methodology　could  be　applied　to　other　complex　glycosphingolipids.","subitem_description_type":"Abstract"}]},"item_2_description_7":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"滋賀大学教育学部紀要, 3, 自然科学, 第51号, pp.35-42","subitem_description_type":"Other"}]},"item_2_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"イトノリ, サキ"}]},{"nameIdentifiers":[{}],"names":[{"name":"タダ, ショウヘイ"}]},{"nameIdentifiers":[{}],"names":[{"name":"スギタ, ムツミ"}]}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"Itonori, Saki"}]},{"nameIdentifiers":[{}],"names":[{"name":"Tada, Shohei"}]},{"nameIdentifiers":[{}],"names":[{"name":"Sugita, Mutsumi"}]}]},"item_2_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"滋賀大学教育学部"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11123267","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1342-9272","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"糸乗, 前"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"多田, 尚平"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"杉田, 陸海"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hydrazine　","subitem_subject_scheme":"Other"},{"subitem_subject":"N-acetylhexosamine","subitem_subject_scheme":"Other"},{"subitem_subject":"Deacetylation","subitem_subject_scheme":"Other"},{"subitem_subject":"Glycosphingolipid　","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"アミノ糖含有スフィンゴ糖脂質のヒドラジン分解 : 分岐糖鎖の結合位置決定への応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アミノ糖含有スフィンゴ糖脂質のヒドラジン分解 : 分岐糖鎖の結合位置決定への応用"}]},"item_type_id":"2","owner":"1","path":["169"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-04-10"},"publish_date":"2009-04-10","publish_status":"0","recid":"1740","relation_version_is_last":true,"title":["アミノ糖含有スフィンゴ糖脂質のヒドラジン分解 : 分岐糖鎖の結合位置決定への応用"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:53:25.042532+00:00"}