@article{oai:shiga-u.repo.nii.ac.jp:00001731, author = {糸乗, 前 and 高橋, 将人 and 青木, 一弘 and 杉田, 陸海}, issue = {50}, journal = {滋賀大学教育学部紀要, 3, 自然科学}, month = {}, note = {Departmental Bulletin Paper, A technique is described for the acetolysis of carbohydrates from glycosphingolipids using HCl/H2O/CH3COOH mixture and a microwave oven. Although acetolysis is important for the determination of the quantitative sugar composition and chemical sugar linkage in glycosphingolipids, the conventional method requires that the sample be reacted for 18 hours, which is a time-consuming step. However, by the microwave method, acetolysis is rapid and complete with only 1 minute exposure. Moreover, some sugar components could be detected only by this method, or showed an improved recovery, especially xylose and its methyl derivative. Microwave-mediated acetolysis is a rapid, quantitative and useful method for structural analysis in the Protostomia glycosphingolipids., 滋賀大学教育学部紀要, 3, 自然科学, 第50号, pp.17-24}, pages = {17--24}, title = {電磁波によるスフィンゴ糖脂質のアセトリシス}, year = {2000} }